Question: (a) Why is it illogical to use a thin stationary phase (0.2 m) in a wide-bore (0.53-mm) open tubular column? (b) Consider a narrow-bore (0.25
(b) Consider a narrow-bore (0.25 mm diameter), thin-film (0.10 μm) column with 5 000 plates per meter. Consider also a wide-bore (0.53 mm diameter), thick-film (5.0 μm) column with 1 500 plates per meter. The density of stationary phase is approximately 1.0 g/mL. What mass of stationary phase is in each column in a length equivalent to one theoretical plate? How many nanograms of analyte can be injected into each column if the mass of analyte is not to exceed 1.0% of the mass of stationary phase in one theoretical plate?
Step by Step Solution
3.34 Rating (169 Votes )
There are 3 Steps involved in it
a A thin stationary phase permits rapid equilibration of analyte between the mobile and stationary p... View full answer
Get step-by-step solutions from verified subject matter experts
Document Format (1 attachment)
878-E-C-E-E-C (2313).docx
120 KBs Word File
