Question: DNA ligase has the ability to relax super-coiled circular DNA in the presence of AMP but not in its absence. (a) What is the mechanism

DNA ligase has the ability to relax super-coiled circular DNA in the presence of AMP but not in its absence.
(a) What is the mechanism of this reaction, and why does it depend on AMP?
(b) How could you determine that super-coiled DNA had in fact been relaxed?

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