Suppose that a replicative DNA polymerase had its 3 exonuclease site 1 5 nm from the polymerase site, rather than the 3 0 nm seen in Klenow fragment How would this change aect the delity of the enzyme Why Describe an additional change that could give this enzyme the same delity as Klenow fragment while retaining the 1 5 nm inter site distance

Question: Suppose that a replicative DNA polymerase had its 3 exonuclease site 1.5 nm from the polymerase site, rather than the 3.0 nm seen in Klenow

Suppose that a replicative DNA polymerase had its 3′ exonuclease site 1.5 nm from the polymerase site, rather than the 3.0 nm seen in Klenow fragment. How would this change aﬀect the ﬁdelity of the enzyme? Why? Describe an additional change that could give this enzyme the same ﬁdelity as Klenow fragment while retaining the 1.5-nm inter-site distance?

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