Question: Early gene-cloning experiments involved insertion at one restriction site in the vector; for example, the insert would have an EcoRI site at each end, and

Early gene-cloning experiments involved insertion at one restriction site in the vector; for example, the insert would have an EcoRI site at each end, and the vector would be opened at an RI site prior to ligation. Under what circumstances would asymmetric cloning be desirable, with the inset having a different restriction site at each end (see Tools of Biochemistry 4A)?

Step by Step Solution

★★★★★

3.35 Rating (164 Votes )

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock

One reason for fixing the orientation in one direction would be a desire to expr... View full answer

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Document Format (1 attachment)

811-M-S-B-C (554).docx

120 KBs Word File

Students Have Also Explored These Related Biochemistry Questions!

Some vectors used in cloning experiments contain bacterial promoters that are adjacent to unique cloning sites. This makes it possible to insert a gene sequence next to the bacterial promoter and...

In the past, most experiments in particle physics involved stationary targets: one particle (usually a proton or an electron) was accelerated to a high energy E, and collided with a target particle...

How does gene cloning produce many copies of a gene?

Under what circumstances would asymmetric cloning be desirable, with the inset having a different restriction site at each end? Early gene-cloning experiments involved insertion at one restriction...

Let A, B be sets. Define: (a) the Cartesian product (A B) (b) the set of relations R between A and B (c) the identity relation A on the set A [3 marks] Suppose S, T are relations between A and B, and...

Beginning in the 1990s, the oil company BP promoted its "green" initiatives, which were designed to highlight the company's environmental practices. It launched an alternative energy division and cut...

2Repor Question 13 Which is involved in the postprocedure nursing care of a child after left-side cardiac catheterization? Encouraging early ambulation Monitoring the insertion site for bleeding...

Describe the inductive and molecular signals involved in the specification of the dorsal-ventral and anterior-posterior axes as found in amphibian embryos. What early embryological experiments showed...

Please read this lecture video texts to answer the questions down below, all the information you need is on this DOCUMENT , do not use outside sources: Lecture 1: And. SPEAKER 1 I won't now want to...

Please use this lecture video texts to answer the questions down below: Lecture 1: And. SPEAKER 1 I won't now want to take up proving the plaintiff's case by expert testimony. The standard or...

D. More About Dimensional Analysis and Conversion Factors here are a number of ways to solve any word problems. In the previous example: There 525 px 0.000625 kg 1000 m 1000 This calculation required...

Calculating Returns and Standard Deviations Based on the following information, calculate the expected return and standard deviation for the two stocks: State of Rate of Return if State Occurs...

TABLE 1 4 . 4 Heifer Sports financial statements \ table [ [ Income statement, 2 0 2 4 Show all images Show all images Show all images done loading

18. A group of 5 boys and 10 girls is lined up in random orderthat is, each of the 15! permutations is assumed to be equally likely. a. What is the probability that the person in the 4th position is...

Free energy changes under intracellular conditions dier markedly from those determined under standard conditions. G = - 30.5 kJ/mol for ATP hydrolysis to ADP and Pi. Calculate G for ATP hydrolysis in...

(a) Consider the following hypothetical metabolic pathway: Under intracellular conditions, the activity of enzyme X is 100 pmol/106 cells/s. Calculate the eect on metabolic ux rate (pmol/106 cells/s)...

Te glucose / glucose-6-phosphate substrate cycle involves distinct reactions of glycolysis and gluconeogenesis that interconvert these two metabolites. Assume that under physiological conditions,...

1. Sade-vanilya faiz oran takas (6 puan) Bir ngiliz irketi olan Britago Corp, Baker Bank ile 6 aylk bir faiz oran takasna girer. Takasn nominal tutar 40 milyon 'dur. demeler ayda bir 90/360 (deme...

Click here to read the eBook: Constant Growth Stocks VALUATION OF A CONSTANT GROWTH STOCK A stock is expected to pay a dividend of $0.50 at the end of the year (i.e., D1 = $0.50), and it should...

FCFE from EBIT An analyst for Remley Company has assembled the following information in local currency units ( LCU ) : EBIT = $ 1 , 6 3 2 Average tax rate = 3 6 % Marginal tax rate = 3 3 %...