Question: Proline has pKa1 = 1.99 and pKa2 = 10.60. Use the HendersonHasselbalch equation to calculate the ratio of protonated and neutral forms at pH =

Proline has pKa1 = 1.99 and pKa2 = 10.60. Use the Henderson—Hasselbalch equation to calculate the ratio of protonated and neutral forms at pH = 2.50. Calculate the ratio of neutral and deprotonated forms at pH = 9.70.

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