An article in Journal of Applied Microbiology ["Use of Response Surface Methodology to Optimize Protease Synthesis by a Novel Strain of Bacillus sp. Isolated from Portuguese Sheep Wool" (2012, Vol. 113(1), pp. 36-43)] described a fractional factorial design with three center points to study six factors (yeast extract, peptone, inoculum concentration, agitation, temperature, and pH) for protease activity. Response units were proteolytic activity per ml (U/ml). The data follow.

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Yeast Peptone (g/L) Inoculum Agitation (грт) Protease Activity (U/ml) Temperature (C) (vol. %) Run (g/L) pH 29.81 1 5 2 34 6. 10 34 15.52 5 1 34 8. 18.23 27.25 4 10 4. 34 34 8. 25.81 10 3 34 37.21 5 4 3 34 6. 22.75 8.01 10 3 34 8. 5 100 34 8. 26.01 10 10 2 100 34 6. 37.21 11 5 4 1 100 34 6. 26.73 100 12 10 4 34 7.4 13 5 3 100 34 6. 36.97 14 10 2 100 34 16.24 100 15 4 3 34 8. 18.15 16 10 4 100 34 6. 25.3 17 5 40 8. 32.6 18 10 2 1 40 6. 47.33 6. 19 40 37.33 20 10 4. 1 40 8. 12.32 21 40 6. 37.7 22 10 3 40 8. 19.01 23 4 40 8. 36.24 24 10 4 3 40 6. 35.88 25 100 40 6. 39.94 2. 2. 2. 2. 2. 4. 2. 2.