Pyruvate was administered to isolated liver cells in the presence of sufficient malonate to block succinate dehydrogenase

Question:

Pyruvate was administered to isolated liver cells in the presence of sufficient malonate to block succinate dehydrogenase completely.

After a time, isocitrate was isolated and found to contain label in both carbon 2 and carbon 5:

14CH2-COO- I HC-COO H14c-COO- T OH

How do you explain this result?

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Biochemistry Concepts And Connections

ISBN: 9780134641621

2nd Edition

Authors: Dean Appling, Spencer Anthony-Cahill, Christopher Mathews

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Question Posted: Nov 06, 2023 03:30 AM

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Pyruvate was administered to isolated liver cells in the presence of sufficient malonate to block succinate dehydrogenase

Question:

14CH2-COO- I HC-COO™ H14c-COO- T OH

Step by Step Answer:

The experiment you described involves the administration of pyruvate to isolated liver cells in the presence of malonate a competitive inhibitor of su...View the full answer

Biochemistry Concepts And Connections

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