You are using a standard microscope with a 0.10NA 4x objective and switch to a 0.65 NA 40X objective. What are the acceptance angles for each? Compare and comment on the values. Which would you use first to locate the target area on of your specimen? (See Figure 26.17.)

Data from Figure 26.17

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to ¹0 P (a) Light Cone (b) α D