What nonparametric test can be used to compare the 16α-OHE 1 percentages on a high-fat diet vs.

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What nonparametric test can be used to compare the 16α-OHE1percentages on a high-fat diet vs. a low-fat diet?


Endocrinology

A study to assess the effect of a low-fat diet on estrogen metabolism recruited 6 healthy women ages 21€“32 [5]. The women were within 5% of their ideal body weight, were not participating in athletics, and were eating a typical American diet. For the first 4 weeks the women were fed a high-fat diet (40% of total calories from fat). They were then switched to a low-fat diet (25% of calories from fat) for 2 months. During the follicular phase of their menstrual cycle (days 5€“7), each woman was given a sugar cube with [3H] E2 (estradiol). This was done once during the high-fat period and again after the woman had been eating the low-fat diet for 2 months. The percentage of orally administered [3H]E2 excreted in urine as 16α-hydroxylated glucoronides is given in Table 9.17.

Table 9.17 Percentage of orally administered [3H]E2 excreted in urine as glucoronides of 16α-OHE1

 High-fat diet Subject Low-fat diet 2.55 1.27 2.92 1.60 3 1.71 0.53 4.00 1.02 0.77 0.74 1.03 0.67

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