Oxygen sensing is important in biological studies of many systems. The variation in oxygen content of sapwood
Question:
Oxygen sensing is important in biological studies of many systems. The variation in oxygen content of sapwood trees was measured by del Hierro and coworkers [ J. Experimental Biology 53 (2002): 559] by monitoring the luminescence intensity of [Ru(dpp)3]2+ immobilized in a sol-gel that coats the end of an optical fiber implanted into the tree. As the oxygen content of the tree increases, the luminescence from the ruthenium complex is quenched. The quenching of [Ru(dpp)3]2+ by O2 was measured by Bright and coworkers [Applied Spectroscopy 52 (1998): 750] and the following data were obtained:
I0/I………………………………%O2
3.6………………………………12
4.8………………………………20
7.8………………………………47
12.2……………………………100
a. Construct a Stern–Volmer plot using the data supplied in the table. For [Ru(dpp)3]2+ kr =1.77 × 105 s–1, what is k q?
b. Comparison of the Stern–Volmer prediction to the quenching data led the authors to suggest that some of the [Ru(dpp)3]2+ molecules are located in sol-gel environments that are not equally accessible to O2. What led the authors to this suggestion?
Step by Step Answer:
The SternVolmer plot of interest is of the form In the above expression k l is the rat...View the full answer
