Modern microscopes are more likely to use a camera than human viewing. This is accomplished by replacing the eyepiece inFigure 35.14with a photo-ocular that focuses the image of the objective to a real image on the sensor of a digital camera. A typical sensor is 22.5 μm wide and consists of 5625 4.0@mm@ wide pixels. Suppose a microscopist pairs a 40× objective with a 2.5× photo-ocular.

a. What is the field of view? That is, what width on the microscope stage, in mm, fills the sensor?
b. The photo of a cell is 120 pixels in diameter. What is the cell€™s actual diameter, in μm?

Transcribed Image Text:

Objective Eyepiece fobj fobj feye foye Tube length L Object The eyepiece acts as a magnifier. It forms an image at infinity that is seen by a relaxed eye. The object is just beyond the focal point. The magnified image of the objective acts as the object for the eyepiece.