A mixture of M. lysodeikticus DNA and rice dwarf virus RNA gave these circular dichroism data in a 1?cm cell:

The pure components gave the following results for solutions containing 1.00 ? 10 ? 4M concentrations of nucleotides per liter:

a. What is eL ? eR for the RNA at 300 nm?

b. What are the concentrations of DNA and RNA in the mixture?

c. If you hydrolyze the mixture to its component nucleotides, will the circular dichroism at 260 nm increase or decrease?

d. Will the absorbance at 260 nm increase or decrease?

e. Will the optical rotatory dispersion increase or decrease?

Transcribed Image Text:

À (nm) 300 280 260 240 AL-AR -0.250 x 10-4 1.16 x 10-4 3.12 x 10-4 -0.24 x 10-4 X(nm) Al-Ar(Dna) | Al-AR(RNA) 300 280 260 240 0.10 x 10-4 -0.50× 10-4 3.00 x 10-4 1.60 x 10-4 0.00 x 10-46.00 x 10-4 -2.20 x 10-4 | 0.00 x 10-4 À (nm) 300 280 260 240 AL-AR -0.250 x 10-4 1.16 x 10-4 3.12 x 10-4 -0.24 x 10-4 X(nm) Al-Ar(Dna) | Al-AR(RNA) 300 280 260 240 0.10 x 10-4 -0.50× 10-4 3.00 x 10-4 1.60 x 10-4 0.00 x 10-46.00 x 10-4 -2.20 x 10-4 | 0.00 x 10-4

Answer rating: 100% (QA)

a From the table provided for RNA at wavelength 300 nm A L A R 050 10 4 Absorbance is given as A cl1 Here is the molar absorptivity at wavelength c is ... View the full answer