1. A solution of a colored solution has an absorption maximum at 460 nm. The absorbance of the solution at 495 nm is 3.5. What color will the solution have to your eyes? What must you do before you can accurately determine its absorbance? (5 points) 2. Solid ATP is very hygroscopic - that is, the powder picks up water from the air. With time, the powder will no longer be pure ATP. how would this affect your accuracy if you were trying to make an exact concentration of ATP using only a balance - would your solution be too concentrated or too dilute? What could you do in order to accurately determine the concentration of the ATP solution? Assume your lab has a spectrophotometer! (5 points) 3. You have a made a solution of NADH. You try to measure the absorbance at 340 nm, but find that the absorbance is too high to measure accurately. You take 0.5ml of this concentrated solution and dilute it to a final volume of 50 ml with water. You measure the absorbance of this dilute solution in a Icm pathlength cuvette, and find the absorbance at 340 nm to be 0.543. . What is the concentration of the undiluted solution? The Molar extinction coefficient for NADH is 6,200 liters/mol cm