1. Chromatography is a separation technique and is based on the distribution of the solute between a stationary and a mobile phase. (a) Explain the difference between the partition coefficient (also named distribution coefficient Ko) and distribution ratio (D). (1 mark) (b) What would the difference be between Ko and D if acetyl salicylic acid (structure below) is separated between an organic and aqueous phase? Please provide a formula for each of the expressions and explain why this would be different if the solute (analyte) was caffeine. (3 marks) (c) Explain what the influence would be on D if the pH is decreased (HINT: use Le Chatelier's principle). (1 mark) OH Gor