1. Eor enzymatic reaction, urease Urea 2NH3 + CO If 5 g/L urease is added,...
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1. Eor enzymatic reaction, urease Urea → 2NH3 + CO₂ If 5 g/L urease is added, the rate of reaction is given as a function of urea concentration in the table below. Curea, mol/L -Lurea, mol/L-s 0.2 1.08 0.02 0.55 0.01 0.38 0.005 0.2 0.002 0.09 In a 0.5 L batch reactor, the initial concentration of urea is 0.1 mol/L, and urease concentration is 0.1 g/L. a). Calculate the time needed to convert 99% of the urea. b). If a competitive inhibitor with a concentration of 0.03 mol/L and K = 0.02 mol/L, calculate the time needed. 2. Lactic acid is produced by a Lactobacillus species cultured in a CSTR. To increase the cell concentration and production rate, most of the cells in the reactor outlet are recycled to the CSTR, such that the cell concentration in the product stream is 10% of cell concentration in the reactor. Find the optimum dilution rate that will maximize lactic acid production rate. [= (au+B)Cc] Mmax= 0.5 h-¹, Ks = 2.0 g/L, a = 0.2 g/g, B=0.1 g/g-h, Cso = 50 g/L, Yxs -0.2 g/g, YP/S = 0.3 g/g 1. Eor enzymatic reaction, urease Urea → 2NH3 + CO₂ If 5 g/L urease is added, the rate of reaction is given as a function of urea concentration in the table below. Curea, mol/L -Lurea, mol/L-s 0.2 1.08 0.02 0.55 0.01 0.38 0.005 0.2 0.002 0.09 In a 0.5 L batch reactor, the initial concentration of urea is 0.1 mol/L, and urease concentration is 0.1 g/L. a). Calculate the time needed to convert 99% of the urea. b). If a competitive inhibitor with a concentration of 0.03 mol/L and K = 0.02 mol/L, calculate the time needed. 2. Lactic acid is produced by a Lactobacillus species cultured in a CSTR. To increase the cell concentration and production rate, most of the cells in the reactor outlet are recycled to the CSTR, such that the cell concentration in the product stream is 10% of cell concentration in the reactor. Find the optimum dilution rate that will maximize lactic acid production rate. [= (au+B)Cc] Mmax= 0.5 h-¹, Ks = 2.0 g/L, a = 0.2 g/g, B=0.1 g/g-h, Cso = 50 g/L, Yxs -0.2 g/g, YP/S = 0.3 g/g
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Related Book For
Biochemistry Concepts and Connections
ISBN: 978-0321839923
1st edition
Authors: Dean R. Appling, Spencer J. Anthony Cahill, Christopher K. Mathews
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