• Calculate the amount of each chemical required to make a 10x stock of TBE in a final volume of 150 mL (Table 7)

• Molecular weight and final concentration (mM) are given. The chemicals are dissolved in distilled water

  Table 7 Preparing 10x TBE

Component	Molecular Weight or Concentration	10x Stock		Final 1x Working Concentration to run gel
		Amount (grams or mL) needed for 150 mL	Stock Concentration to make
Tris Base	121.14 g/mol		890 mM	89 mM
Boric Acid	1M		890 mM	89 mM
EDTA*	372.24 g/mol		20 mM	2 mM

*Ethylenediaminetetraacetic acid