Calculate the amount of each chemical required to make a 10x stock of TBE in a final
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Question:
Calculate the amount of each chemical required to make a 10x stock of TBE in a final volume of 150 mL (Table 7)
Molecular weight and final concentration (mM) are given. The chemicals are dissolved in distilled water
Table 7 Preparing 10x TBE
Component | Molecular Weight | 10x Stock | Final 1x Working Concentration to run gel | |
Amount | Stock Concentration to make | |||
Tris Base | 121.14 g/mol | 890 mM | 89 mM | |
Boric Acid | 1M | 890 mM | 89 mM | |
EDTA* | 372.24 g/mol | 20 mM | 2 mM |
*Ethylenediaminetetraacetic acid
Related Book For
Essentials of Marketing Research
ISBN: 978-1305263475
6th edition
Authors: Barry J. Babin, William G. Zikmund
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