1. For feed media to a bioreactor, the dissolved oxygen concentration is proportional to that in...

 

Transcribed Image Text:

1. For feed media to a bioreactor, the dissolved oxygen concentration is proportional to that in the air surrounding the medium. The oxygen concentration will reach an equilibrium between the medium and the surrounding air. At 37 C and 1.0 atm of pressure, air is 21.2% O, 0.15 % CO and 78.65 % N. a. Determine the mass and molar concentrations of each of the gases in the air. b. Find the concentration of dissolved O in the culture medium. Assume that culture medium has the same O solubility properties as water and that the concentration of O in the air is not affected by the transport of oxygen into the medium. The Henry's constant is 5.21 x 104 atm/mole fraction of O in water. c. Prior to use, the medium is most often stored at 4 C where Henry's constant is 2.95 x104. How will this temperature change affect the concentration of dissolved oxygen in the medium? Show the calculations that support your answer. 1. For feed media to a bioreactor, the dissolved oxygen concentration is proportional to that in the air surrounding the medium. The oxygen concentration will reach an equilibrium between the medium and the surrounding air. At 37 C and 1.0 atm of pressure, air is 21.2% O, 0.15 % CO and 78.65 % N. a. Determine the mass and molar concentrations of each of the gases in the air. b. Find the concentration of dissolved O in the culture medium. Assume that culture medium has the same O solubility properties as water and that the concentration of O in the air is not affected by the transport of oxygen into the medium. The Henry's constant is 5.21 x 104 atm/mole fraction of O in water. c. Prior to use, the medium is most often stored at 4 C where Henry's constant is 2.95 x104. How will this temperature change affect the concentration of dissolved oxygen in the medium? Show the calculations that support your answer.

Answer rating: 100% (QA)

a Mass and Molar Concentrations of Gases in Air Total moles of air We can assume 1 mole of air for simplicity Nitrogen N Mole fraction XN 07865 Moles ... View the full answer