1. In a TLC analysis why do some spots move up the plate faster than other spots. 2. Calculate the Rr value of the material on the TLC plate below. 3. Why is it important that the initial spot of the material not be at the very bottom of the plate? 4. Why is ink not used to make the initial markings on the plate? Once a plate has been developed, the spots may be visualized in different ways. 5. Give an example of destructive visualization... 6. Give an example of nondestructive visualization... 7. What happens when your initial spot is too concentrated? 8. What happens when spots are too close to the edge of the plate? 9. If an unknown has an R value close to two knowns, how could cospotting help identify it? 10. Changing the developing solvent to a more polar solvent will do what to the Revalues