10)Examine each of the errors below, discuss how the error would affect the separation. Describe how each error would impact the efficacy of the purification (better separation, worse separation, no effect). (Error) a) Overbunched cotton ball at bottom of column b) The silica gel is not level when the sample is loaded onto the column c) Half as much silica gel was used as was indicated in the procedure d) Smaller fractions are collected (less volume in each test tube with test tubes switched more frequently)