12. Prepare a 350 ml solution that is 25 mM Tris, 2.5 mM EDTA, pH 8.0. The MW of Tris is 121.1. MW of EDTA is 187. You would start by measuring 275 mls of water and then adding: MV = M V2 Tris EDTA then you would need to: 7 Unit 1: Basic skills - week 2 13. A certain protocol calls for 1L of a solution that is 1.5 % (w/v) NaCl 7% (w/v) dry milk, and 0.02% (v/v) antifoam agent. To prepare this solution one would use Naci Dry milk antifoam agent