4. In a Bradford experiment, you make four dilutions (1:5, 1:10; 1:100 and 1:1000) of an...

   

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4. In a Bradford experiment, you make four dilutions (1:5, 1:10; 1:100 and 1:1000) of an unknown stock protein. Using a BSA control, you produce the standard curve below. The absorbance at 595nm of the 1:10 dilution is 0.7. What is the concentration of the stock solution? 0.8 0.7 0.6 0.5 0.4 Net Absorbance (595nm) 0.3 0.2 0.1 0 200 400 600 1000 BSA Concentration (g/ml) 800 1200 1400 1600 5. In a Bradford experiment, you make the following four dilutions an unknown stock protein. Tube A1:10 Tube B 1:100. Tube C=1:1000 Tube D 1:5000 = Using a BSA control, you produce the standard curve above. The absorbance at 595nm of the 1:100 dilution is 0.2. What is the concentration of tube B? 4. In a Bradford experiment, you make four dilutions (1:5, 1:10; 1:100 and 1:1000) of an unknown stock protein. Using a BSA control, you produce the standard curve below. The absorbance at 595nm of the 1:10 dilution is 0.7. What is the concentration of the stock solution? 0.8 0.7 0.6 0.5 0.4 Net Absorbance (595nm) 0.3 0.2 0.1 0 200 400 600 1000 BSA Concentration (g/ml) 800 1200 1400 1600 5. In a Bradford experiment, you make the following four dilutions an unknown stock protein. Tube A1:10 Tube B 1:100. Tube C=1:1000 Tube D 1:5000 = Using a BSA control, you produce the standard curve above. The absorbance at 595nm of the 1:100 dilution is 0.2. What is the concentration of tube B?

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To determine the concentration of the stock solution in the first scenario where the abso... View the full answer