Question: A protein is being separated on a chromatography column. The protein has been found to obey the Langmuir isotherm, with Stot = 300 g/ml and

A protein is being separated on a chromatography column. The protein has been found to obey the Langmuir isotherm, with Stot = 300 g/ml and Keq = 10 ml/mg. The concentration of the protein in the 3 L feed to the column is 2 mg/mL. The column flow rate is 2.0 liters/min. The column is 50 cm in diameter and 100 cm long. The void fraction of the chromatography bed is 0.35. Determine the elution profile.

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