Question: Protein A and protein B were separated using affinity chromatography. Lab studies shown that capacity factors for the protein are 0.81 for A-Chain and 1.17

Protein A and protein B were separated using affinity chromatography. Lab studies shown that capacity factors for the protein are 0.81 for A-Chain and 1.17 for B-Chain. It follows Van Deemter equation and the values of A, B and C are 2.3 10-9 m2/s, 1.4 s and 5.4 10-5 m respectively. Two columns of inner diameter 26 cm are available for the process. One is 1.3 m high , other is 0.6 m high. (a) What is the minimum HETP. Find by graphical method? (b)If larger column is used with a liquid flow rate of 0.29 lit/min, will the two proteins be completely separated?

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