A protein sample complex consists of two proteins, a smaller protein, X, and a larger protein,...

 

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A protein sample complex consists of two proteins, a smaller protein, X, and a larger protein, Y. Protein X is composed of two polypeptide chains linked by disulfide bonds. Protein Y is composed of three polypeptide chains linked by disulfide bonds. The complex is analyzed by native PAGE, reducing SDS-PAGE and non-reducing SDS-PAGE. Native PAGE does not include sodium dodecyl sulfate, or SDS. Reducing SDS-PAGE uses both SDS and a reducing agent in the buffer. Non-reducing SDS-PAGE uses SDS, but no reducing agent. Match each gel pattern to an experimental method. The first lane in each gel is a molecular weight ladder. Native PAGE Non-reducing SDS-PAGE Reducing SDS-PAGE Answer Bank Answer Bank 250 kDa 1000 kDa 150 kDa 500 kDa 100 kDa 250 kDa %3 60 kDa 100 kDa 50 kDa 50 kDa 30 kDa 30 kDa 250 kDa 150 kDa 100 kDa 60 kDa 50 kDa 30 kDa What are the molecular weights of the protein X polypeptides? What are the molecular weights of the protein Y polypeptides? A protein sample complex consists of two proteins, a smaller protein, X, and a larger protein, Y. Protein X is composed of two polypeptide chains linked by disulfide bonds. Protein Y is composed of three polypeptide chains linked by disulfide bonds. The complex is analyzed by native PAGE, reducing SDS-PAGE and non-reducing SDS-PAGE. Native PAGE does not include sodium dodecyl sulfate, or SDS. Reducing SDS-PAGE uses both SDS and a reducing agent in the buffer. Non-reducing SDS-PAGE uses SDS, but no reducing agent. Match each gel pattern to an experimental method. The first lane in each gel is a molecular weight ladder. Native PAGE Non-reducing SDS-PAGE Reducing SDS-PAGE Answer Bank Answer Bank 250 kDa 1000 kDa 150 kDa 500 kDa 100 kDa 250 kDa %3 60 kDa 100 kDa 50 kDa 50 kDa 30 kDa 30 kDa 250 kDa 150 kDa 100 kDa 60 kDa 50 kDa 30 kDa What are the molecular weights of the protein X polypeptides? What are the molecular weights of the protein Y polypeptides?

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Ans Molecular weight of the protein X i... View the full answer