A sample of an unknown peptide undergoes a single round of Edman degredation, which liberates ATZ-Gly. Another sample of the peptide is treated with trypsin,
A sample of an unknown peptide undergoes a single round of Edman degredation, which liberates ATZ-Gly. Another sample of the peptide is treated with trypsin, and a third sample is treated with chymotrypsin. The table below shows the fragments. Complete parts (a) and (b) below.
b. write the sequence of the peptide.
Tryptic fragments Chymotryptic fragments Ma Arg-Asn-Tyr Ala-Val-Thr-Cys-Asn-Gln-Lys-Trp Trp-Ser Gly-Val-Tyr-Arg Asn-Tyr-Ala-Val-Thr-Cys-Asn-Gln-Lys Ser Gly-Val-Tyr a) Line up the fragments below so that fragments with the same sequences overlap. Drag each fragment to the appropriate location. Asn-Tyr-Ala-Val-Thr-Cys-Asn-Gin-Lys Gly-Val-Tyr Ala-Val-Thr-Cys-Asn-Gln-Lys-Trp Gly-Val-Tyr-Arg Ser Trp-Ser Arg-Asn-Tyr
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Authors: Darrell Ebbing, Steven D. Gammon
9th edition
978-0618857487, 618857486, 143904399X , 978-1439043998
