Question: A single - molecule microscopy slide is prepared with 9 5 0 molecules each of magenta, teal, crimson, and amber fluorescent proteins ( MFP ,

A single-molecule microscopy slide is prepared with 950 molecules each of magenta, teal,
crimson, and amber fluorescent proteins (MFP, TFP, CrFP, and AFP). At a concentration of
3 nM antibody, results show 870 molecules of MFP,12 of TFP,3 of CrFP, and 6 of AFP are
bound. a. What is the antibody specificity for MFP? b. Determine the KD of the
MFPantibody interaction.

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