Question: ( a ) The chromatogram below shows a Reversed - Phase Liquid chromatography ( RPLC ) gradient to separate a mixture over 4 0 minutes.
a The chromatogram below shows a ReversedPhase Liquid chromatography RPLC
gradient to separate a mixture over minutes. The mobile phase consisted of
Acetonitrile
All peaks eluted between min and min.
i Using the chromatographic details given above, determine if isocratic or gradient
elution is more suitable for the separation of this mixture.
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