Question: ( a ) The chromatogram below shows a Reversed - Phase Liquid chromatography ( RPLC ) gradient to separate a mixture over 4 0 minutes.

(a) The chromatogram below shows a Reversed-Phase Liquid chromatography (RPLC)
gradient to separate a mixture over 40 minutes. The mobile phase consisted of
H2O? Acetonitrile (5-95%).
All peaks eluted between 17.2min and 34.7min.
(i) Using the chromatographic details given above, determine if isocratic or gradient
elution is more suitable for the separation of this mixture.
 (a) The chromatogram below shows a Reversed-Phase Liquid chromatography (RPLC) gradient

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