Advanced Separation Processes

The experimental pulse response peak is reported for 100l-injection of 2g/1 lysozyme (Mr15kDa) in a 1cm diameter, 10cm long SP-Sepharose-FF column (100m particle size) with a mobile phase containing 1.5MNaCl in 10mMNa2HPO4 buffer adjusted to pH 6.5 with phosphoric acid and flowing at a rate of 4ml/min. The data are reported as mAU versus eluted volume in ml. Determine the HETP using three Graphical (Mid-Height Width, Baseline \& Area) and Moment methods and compare the results with Exponentially Modified Gaussian (EMG) method. Approximate numbers for mAU and V can be taken from the response peak for simplification