Question: An antibody with two equivalent binding sites is present at a concentration of 2 0 nM . If an antigen is present at 4 0

An antibody with two equivalent binding sites is present at a concentration of 20 nM. If an
antigen is present at 40 nM and the KD for binding either end is 4 nM, what fraction of antibody is singly
bound at steady state? What fraction is doubly bound at steady state? Assume antigen is in excess

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