Question: An E . coli F - strain has the following genotype: S UR F E C . Three different fr strains, all carrying S *

An E. coli F- strain has the following genotype: S UR F E C. Three different fr strains, all carrying S* U+R+ I*E and C* markers and all having lambda phages integrated at the same specific site in their chromosomes are mated with the F strain in separate matings. The interrupted-mating results are given below. The numbers indicate time (in minutes) when different donor markers appeared in F cells after conjugation began. Assume that the E. coli map consists of 100 minutes and gene C is mapped at 80 minutes.The positions of Hfr 2, the gene C, and the polarity of Hfr 2 are given in the accompanying map.MarkersHfr 1Hfr 2Hfr 3R1020405U255E6045205570.(a) Give the gene order, starting from C and going clockwise. (3 pt.)0 min1007525Hfr271.(b) What is the location of the origin of Hfr 1? Hfr 3?(1 pt)72.(c) What is the location of the / marker? The S marker? (1 pt)73.(d) What is the location of the lambda phage in these strains? )Hint: The lambda has to be present in all transfers to cause lysis)(1 pt)

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