Question: An E . coli F - strain has the following genotype: S UR F E C . Three different fr strains, all carrying S *
An E coli F strain has the following genotype: S UR F E C Three different fr strains, all carrying S UR IE and C markers and all having lambda phages integrated at the same specific site in their chromosomes are mated with the F strain in separate matings. The interruptedmating results are given below. The numbers indicate time in minutes when different donor markers appeared in F cells after conjugation began. Assume that the E coli map consists of minutes and gene C is mapped at minutes.The positions of Hfr the gene C and the polarity of Hfr are given in the accompanying map.MarkersHfr Hfr Hfr RUEa Give the gene order, starting from C and going clockwise. pt minHfrb What is the location of the origin of Hfr Hfr ptc What is the location of the marker? The S marker? ptd What is the location of the lambda phage in these strains? Hint: The lambda has to be present in all transfers to cause lysis pt
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