Question: Answer both parts step by step please. 1. A Lineweaver-Burke plot for the action of the enzyme alkaline phosphatase on the substrate molecule PNPP (para-nitrophenylphosphate)

Answer both parts step by step please. 1. A Lineweaver-Burke plot

Answer both parts step by step please.

1. A Lineweaver-Burke plot for the action of the enzyme alkaline phosphatase on the substrate molecule PNPP (para-nitrophenylphosphate) yielding the product para-nitrophenolate gives a y-intercept of 100.24 min mM and a slope of 0.3374 min. (a) Determine V max in mM sect and Ky in MM (b) If the concentration of enzyme is 5.0 x 10-6 mM, what is the value of kcat in sec-l

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