9. In three separate site-directed mutagenesis experiments, scientists altered a Lys residue in corn PEP carboxylase...

 

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9. In three separate site-directed mutagenesis experiments, scientists altered a Lys residue in corn PEP carboxylase to an Asn, Glu, or Arg. Which substitution would you expect to have the least effect on enzymatic activity? Which substitution would have the greatest effect? Explain 10. A 24-residue peptide called Pandinin 2, isolated from scorpion venom, was found to have both antimicrobial and hemolytic properties. The sequence of the first 18 residues of this peptide is shown below. The peptide forms an amphipathic helix in which nonpolar amino acids are on one side of helix and polar or charged amino acids are on the other side of the helix. Examine the sequence below and predict which amino acid side chains are likely to be found on the nonpolar side of the amphipathic helix. a. Propose a hypothesis that explains why the peptide is able to lyse bacteria and red blood cells. 9. In three separate site-directed mutagenesis experiments, scientists altered a Lys residue in corn PEP carboxylase to an Asn, Glu, or Arg. Which substitution would you expect to have the least effect on enzymatic activity? Which substitution would have the greatest effect? Explain 10. A 24-residue peptide called Pandinin 2, isolated from scorpion venom, was found to have both antimicrobial and hemolytic properties. The sequence of the first 18 residues of this peptide is shown below. The peptide forms an amphipathic helix in which nonpolar amino acids are on one side of helix and polar or charged amino acids are on the other side of the helix. Examine the sequence below and predict which amino acid side chains are likely to be found on the nonpolar side of the amphipathic helix. a. Propose a hypothesis that explains why the peptide is able to lyse bacteria and red blood cells.

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