Calculate the absorption coefficient.

Calculate the absorption coefficient at the max values for each of fluorescein, NADH, fully reduced and fully oxidized cytochrome c. Use a pathlength of 1.00cm for these calculations. (Show the step of calculation) a). fluorescein: use 200L of 90M fluorescein with 800L of 40mM phosphate buffer (pH=7). Since =490nm has a max absorbance 0.87 b). NADH: 0.250mM NADH. Since =340nm has a max absorbance 1.361 c). fully reduced cytochrome: use 900L of 0.300mg/mL cytochrome c solution mixed with 100L of 60.0mM ascorbic acid. Since =550nm has a max absorbance 0.56 d). Fully oxidized cytochrome c: use 900L of 0.300mg/mL cytochrome c solution mixed with 100L of 60.0mM ferricyanide. Since =530nm has a max absorbance 0.27