Calculate the absorption coefficient at the max values for each of fluorescein, NADH, fully reduced and fully oxidized cytochrome c. Use a pathlength of 1.00cm for these calculations. (show the step of calculation) a). fluorescein: use 200L of 90M fluorescein with 800L of 40mM phosphate buffer(pH=7). Since = 490nm has an absorbance 0.87

b). NADH: 0.250mM NADH.Since =340nm has an absorbance 1.361

c). fully reduced cytochrome: use 900L of 0.300 mg/mL cytochrome c solution mixed with 100L of 60.0mM ascorbic acid. Since =550nm has an absorbance 0.56

d). Fully oxdized cytochrome c: use 900L of 0.300 mg/mL cytochrome c solution mixed with 100L of 60.0mM ferricyanide. Since =530nm has an absorbance 0.27