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magnesium ions are then titrated with Eriochrome Black T as the indicator. Note that the concentration of the magnesium solution is not important. Procedure: Preparation of EDTA Solution. Calculate the mass of EDTA, Na2H2Y-2H20 (molar mass = 372.24 g/mol), to prepare 500mL of an approximately 0.01M EDTA solution. Weigh it out and transfer it into a clean 500-mL volumetric flask. Add distilled water less than 400 mL and gently shake the volumetric flask until all EDTA salts are completely dissolved. Add additional distilled water to the 500-ml mark and shake it again. Preparation of MgY2- Spike Solution. Calculate the mass of 1.0 mmol (0.0010 mol) MgSO4.7H20; weigh it out; and transfer it into a 250-ml beaker. (Note: sometimes the stockroom provides MgCl2.2H20. Carefully check the chemical label to calculate the correct amount.) Also calculate the mass of 1.0 mmol (0.0010 mol) of EDTA; weigh it out; and transfer it into the same beaker containing MgSO4.7H20 (or MgCl2:2H20). Dissolve them completely with about 30 mL of distilled water and quantitatively transfer the solution into a 50-ml volumetric flask. Add distilled water to the 50-mL mark and gently shake the volumetric flask. Label it as "MgY2-Spike". Preparation of 6M HCl and 6M NaOH solutions. Calculate the volume of the hydrochloric acid stock solution (HCI; Note 1) to prepare 25.0 mL of 6.0 M HCI. Clean a 50-ml (or 40-mL) beaker; add - 5.0 mL of distilled water to it; then add the calculated volume of the stock HCl to the beaker where 5 mL of distilled water is contained; and finally dilute this solution to 25.0 mL. Label this beaker as 6.0 M Hydrochloric acid". Calculate the volume of 50% sodium hydroxide stock solution (NaOH; Note 3) to prepare 25.0 mL of 6.0 M NaOH. Then repeat the same procedure above to dilute 50% NaOH to 6.0 M NaOH