Calculate the transformation efficiencies for each group. Show me one example calculation and organize your data in
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Calculate the transformation efficiencies for each group. Show me one example calculation and organize your data in a table. Is there a trend in transformation efficiencies? Why would transformation efficiencies differ between APV+I, V+I, APV, and positive pUC19 control? How would you expect them to differ between groups?
Volume plated= 50,100,200 ul Total transformation volumes=310ul pg DNA used= 101.7ng = 101700pg
I've been using this formula, and here is my work for one. I'm I doing this correctly?
APV+1, 50ul, W:15
number of colonies/Volume plated (ul) * total transformation/pg DNA used * 10^6pg/ug = 914/1ug DNA
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