Question: calculate the vakues for A and HA Phosphate Buffer, 0.15M,pH 7.1, 2.0L This document will provide the method and calculations for making 2.0L of a

calculate the vakues for A and HA

Phosphate Buffer, 0.15M,pH 7.1, 2.0L This document will provide the method and calculations for making 2.0L of a 150mM phosphate buffer with a pH value of 7.1. Data [Buffer] =0.2M pKa of phosphoric acid: 7.2 Total concentration of phosphate: 150mM Molecular weight of Na2HPO4:142g/mole Molecular weight of NaH2PO4:120g/mole Calculations The ratio of phosphate to acid can be calculated using the Henderson-Hasselbalch equation. pH=pKa+log(HAA)

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