Question: Calculate what a Scatchard plot would look like for a system where there was one tight binding site (K1 = 107 M1) and one weaker

Calculate what a Scatchard plot would look like for a system where there was one tight binding site (K1 = 107 M1) and one weaker binding site (K2 = 2106 M1). Plot v/[Afree] vs v. The simplest way to do this is to let the free A concentration loop from 0 to some maximum value (where you think the system would be close to saturated), and evaluate v and v/[Afree] at each choice

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock blur-text-image

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Mathematics Questions!

Dialysis may be used to study the binding of small molecules to macromolecules, such as an inhibitor to an enzyme, an antibiotic to DNA, and any other instance of cooperation or inhibition by small...

Figure 18-7 is a Scatchard plot for the addition of 0-20 nM antigen X to a fixed concentration of antibody P (P 0 = 10 nM). Prepare a Scatchard plot from the data in the table and find K for the...

Consider a protein with four equivalent and independent binding sites for a ligand. A ) Find the intrinsic dissociation constant, Kd , given fractional saturation point of the enzyme with four...

am uploading a picture of my work. My answer of 27.2kJ/mol was marked wrong. f=0.5Ap=[L](f11)=50pM(i,4,5)[I]=K=50pM Here is a cleaned up version of what you should have gotten from the last part:...

We conducted a protein-ligand binding assay and plotted the data in the form of a Scatchard plot. Here is the plot (where [L] is expressed in nM ) Given this plot: How many ligand binding sites are...

This is a Scatchard plot for the formation of protein-ligand complex where there is only one binding site. The protein concentration is 3.1M. The data at 20C is in black and the data at 37C is in red...

lons bind to a given protein in a 1:1 complex. The data obtained for ligand binding is: By making a suitable plot, find KD for this binding process: Give your answer to two significant figures. Hint:...

Someone was studying protein-ligand binding with a protein concentration of 7.5M. The ligand binding data is as follows: Make a Scatchard plot (Ln vs n~) and upload this plot here: How many ligand...

This is a Scatchard plot for the formation of protein-ligand complex where there is only one binding site. The protein concentration is 2.2M. The data at 20C is in black and the data at 37C is in red...

Prepare the necessary adjusting journal entries on December 31 for each situation. (b) Prepare the necessary reversing journal entries on January 1 of the subsequent year. 1..Company Q paid for a...

Words given precise definitions in physics are sometimes used in popular literature in interesting ways. For example, a rock falling from the top of a cliff is said to be gathering force as it falls...

______ requires certain minimum information be reported on the face of the income statement, while Blank______ does not have minimum requirements. Multiple choice question. EITF; AASB US GAAP; IAS US...

What substantive procedures can we do over debt and equity capital (i.e. confirmations, etc)? Know in detail how these procedures are done, from what the client prepares to what the auditor does.