Question: Figure 18-7 is a Scatchard plot for the addition of 0-20 nM antigen X to a fixed concentration of antibody P (P 0 = 10

Figure 18-7 is a Scatchard plot for the addition of 0-20 nM antigen X to a fixed concentration of antibody P (P₀ = 10 nM). Prepare a Scatchard plot from the data in the table and find K for the reaction P + X ⇌ PX . The table gives measured concentrations of unbound X and the complex PX. It is recommended that the fraction of saturation should span the range ~0.2 - 0.8. What is the range of the fraction of saturation for the data?

Figure 18-7

[X] (nM) 0.731 1.22 2.87 0.120 0.192 0.296 0.450 6.7 7.5 8.45 4.6 5.54 0 3.61

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