Question: Complete each part A-C explain and show work ENZYME INHIBITION STUDIES HOMEWORK 1. The data for the inhibition studies of the papain-catalyzed hydrolysis of p-nitrophenyl
Complete each part A-C
explain and show work
ENZYME INHIBITION STUDIES HOMEWORK 1. The data for the inhibition studies of the papain-catalyzed hydrolysis of p-nitrophenyl hippurate by N-benzoylamino acetaldehyde at a concentration of 3.3 x 10-SM is given below: a. Construct a Lineweaver-Burke plot using the data below. b. Determine the Km and Vmax for the uninhibited and inhibited enzyme c. Determine the type of inhibition imposed by N-benzoylamino acetaldehyde. [S] MM Velocity (uninhibited) Velocity (inhibitor A) Velocity (inhibitor B) (x10) ukat (x10)ukat (x10) ukat 0.333 1.65 1.05 0.794 0.893 0.4 1.86 1.21 0.5 2.13 0.666 2.49 1.43 1.74 2.22 1.02 1.19 1.43 2.99 1.0 2.0 3.72 3.08 1.79
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