CTCF proteins binds to specific DNA sequence motif and are important to form chromosome loops. Two motifs are paired to form a loop by the CTCF protein. However, the two motif can be paired to form loops only when...

both motifs are pointing away from each other, orienting in opposite directions

both motifs are pointing in the same direction

both motifs are pointing toward each other

directionality of the two motifs is not important to form a loop