Question: explain step by step please 3. Many proteins are sensitive to divalent cations and often chelating agents are included in protein isolation solutions to bind
3. Many proteins are sensitive to divalent cations and often chelating agents are included in protein isolation solutions to bind divalent cations, lowering their concentration, and removing them from proteins. Two common agents used for this purpose are EDTA (Ethylenediaminetetraacetic acid) and EGTA (Ethyleneglycol-bis-( aminoethyl ether)-N,N,N',N'-tetraacetic acid). (c) To make up 1L of a 0.5M solution of EDTA starting with the free acid, approximately how much 10MNaOH will have to be added to adjust the pH to 7.0 ? Do you expect this solution to have a pH-buffering capacity? Explain
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