Question: Explain the steps to solve the problem: The following data were obtained from the action of an ATPase on ATP at 20 C, with an
Explain the steps to solve the problem:
The following data were obtained from the action of an ATPase on ATP at 20 C, with an ATPase concentration of 20 nmoldm-3. Calculate the Michaelis constant, the maximum velocity, the catalytic constant, and the catalytic efficiency.
| ATP (moldm-3) | v (moldm-3s-1) |
| 0.60 | 0.81 |
| 0.80 | 0.97 |
| 1.4 | 1.30 |
| 2.0 | 1.47 |
| 3.0 | 1.69 |
CORRECT ANSWERS
KM=0.84 moldm-3
=5.87x106 moldm-3s-1
vmax=0.99 moldm-3s-1
kcat=4.95x107s-1
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