Question: Figure below is experimental data on the solubility, primary nucleation threshold (PNT) and secondary nucleation threshold (SNT) for a protein, alpha amylase. I wish

Figure below is experimental data on the solubility, primary nucleation threshold (PNT)

Figure below is experimental data on the solubility, primary nucleation threshold (PNT) and secondary nucleation threshold (SNT) for a protein, alpha amylase. I wish to perform a seeded-batch crystallization of alpha amylase at 25 C, pH 7, 7.5 wt. % sodium sulfate with no nucleation. What is the maximum starting concentration of alpha amylase and corresponding maximum crystal yield I can achieve from this crystallization? 35 + Solubility -1-hr PNT + 1-hr SNT 30 25 20 15 10 5 10 15 20 25 Sodium sulfate wt % Solubility-supersolubility diagram for a-amalase in 20 mM sodium phosphate buffers at pH 7 and 25C. ju Bu) ouoo asejkuy

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