Question: For a protein containing no -sheet secondary structure the CD spectrum gives [] 222 = -29,750 deg.cm 2 .decimole -1 . Given from reference spectra

For a protein containing no -sheet secondary structure the CD spectrum gives [Ө] 222 = -29,750 deg.cm 2 .decimole -1 . Given from reference spectra that 100% helix has a [Ө] 222 = -36,000 deg.cm 2 .decimole -1 and 100% coil has a [Ө] 222 = +3,000 deg.cm 2 .decimole -1 .

a). What is the percentage alpha-helical content of the protein?

b.) The protein and a short duplex oligonucleotide are added at an equimolar concentration in a buffer containing 100 mM NaCl and then in a buffer containing 1 M NaCl, the two samples are analyzed by CD. The spectra in the 250 to 280 nm region differ considerably between the samples but the spectrums in the 200 – 230 nm range are similar. What hypothesis can you form concerning the protein

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