Question: For a protein containing no -sheet secondary structure the CD spectrum gives [] 222 = -29,750 deg.cm 2 .decimole -1 . Given from reference spectra

For a protein containing no -sheet secondary structure the CD spectrum gives [] 222 = -29,750 deg.cm 2 .decimole -1 . Given from reference spectra that 100% helix has a [] 222 = -36,000 deg.cm 2 .decimole -1 and 100% coil has a [] 222 = +3,000 deg.cm 2 .decimole -1 .

a). What is the percentage alpha helical content of the protein? Show your working.

b.) The protein and a short duplex oligonucleotide are added at equimolar concentration in a buffer containing 100 mM NaCl and then in a buffer containing 1 M NaCl, the two samples are analysed by CD. The spectra in the 250 to 280 nm region differs considerably between the samples but the spectrums in the 200 230 nm range are similar. What hypothesis can you form concerning the protein

Step by Step Solution

There are 3 Steps involved in it

1 Expert Approved Answer

Step: 1 Unlock blur-text-image

Question Has Been Solved by an Expert!

Get step-by-step solutions from verified subject matter experts

Step: 2 Unlock

Step: 3 Unlock

Students Have Also Explored These Related Chemistry Questions!

For a protein containing no -sheet secondary structure the CD spectrum gives [] 222 = -29,750 deg.cm 2 .decimole -1 . Given from reference spectra that 100% helix has a [] 222 = -36,000 deg.cm 2...

The behavior of receptor X (XR), a transmembrane protein present in the plasma membrane of mammalian cells, is being investigated. The protein has been engineered as a fusion protein containing the...

1. Consider a small protein containing 101 amino acid residues. The protein would have 100 peptide bonds about which rotation can occur. Assume three orientations are possible about each of these...

The graph shows the titration curve for a protein containing 124 amino acids with 16 basic and 20 acidic substituents. The curve is smooth without clear breaks because 29 groups are titrated in the...

Three levels of protein structure are primary, secondary and tertiary. a. Describe the primary structure of a typical protein containing one polypeptide chain. Name the type of chemical bonding...

The yeast two-hybrid method is a powerful molecular genetic method to identify a protcin(s) that interacts with a known protein or protein domain. You have isolated the glucocorticoid receptor (GR)...

6. (6 points) (Chapter 6, problem 6 in textbook) Consider a small protein containing 101 amino acid residues. The protein will have 200 bonds about which rotation can occur. Assume that three...

Our body has the need to eat protein containing foods because: we need essential amino acids that can only be provided by foods our bodies are not able to make any amino acids for protein structure...

Hemoglobin is a protein containing iron that facilitates the transport of oxygen in red blood cells. Hemoglobin levels in females are normally distributed and low levels are often associated with...

Nutrus Indus Laboratories is a 56 year old Indian pharmaceutical company engaged in the manufacture of bulk drugs and formulations. It focuses on gastroenterology, cardiology, neuro-psychiatry,...

Suppose we are interested in estimating the model: y = 0 + 1x1 + u. Suppose E(u|x1) = 0 and V ar(ux) = 2. We collected a random sample following the above model with size N. (a) Suppose we use only...

Find the three internal angles of the triangle with vertices: A(3, 1, -2), B(5, 2, -1), and C(4, 3, -3)

If all assets and liabilities of a firm s foreign subsidiary are translated into the parent s currency at the current exchange rate ( the rate in effect at the date of the balance sheet ) , the...

Q1. how is there numbers less than 200 in the third octet if the mask is /21 http://ccna.exampointers.com/sub.php 95.45.204.0 /21 Subnet Network First Usable IP Last Usable IP Broadcast 0 95.45.0.0...

What are the skills you need to be an effective listener? [LO-4]

Why is it important to also connect in person when using technology to communicate? [LO-7]

The memo inferred that the economic downturn will have a greater affect on the companys bottom line then we previously assumed, this was the worse news we could of gotten.