Question: For a protein with two ligand binding sites the binding polynomial has the general form: Q=[P](1+2k1[L]+k1k2[L]2) where [P] is the unbound protein concentration and [L]

For a protein with two ligand binding sites the binding polynomial has the general form: Q=[P](1+2k1[L]+k1k2[L]2) where [P] is the unbound protein concentration and [L] is the free ligand concentration. The equilibrium constants are defined as: k1=[PL][P][L] and k2=[PL2][PL][L]

A. Assume k1=k2=k=10 and [L]=0.15. Calculate the fraction of sites bound fB.

Answer for A is not 0.84

B. Consider a model where k1=k and k2=k If k = 10 and [L] = 0.15 as before, calculate the binding fraction is =0.1

C.Repeat the calculation in part A only assume [L]=2.

D.Repeat the calculation in part B only assume [L]=2

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