For part 2e what was the initial pH ? What was the final pH ? How was the rate of the reaction affected by the increase in pH ? final pH Initial pH Did this change of pH in part 2e affect the configuration of the active site? What amino acid residue in catalase would be changed by a more basic pH? Show the reaction of a residue with sodium hydroxide. (Hint: Draw a salt bridge) Complete the following table of the rates of reaction of catalase (the enzyme) with hydrogen neroxide (the substrate). Express in fraction form the ratio of Volume A/Volume B. Express in fraction form the ratio of drops of substrate in A/drops of substrate in B. How do the fractions compare? 3.43.5 Compare the volume of O2 collected at 0C to the standard (35C) Explain the difference. For part 2e what was the initial pH ? What was the final pH ? How was the rate of the reaction affected by the increase in pH ? final pH Initial pH Did this change of pH in part 2e affect the configuration of the active site? What amino acid residue in catalase would be changed by a more basic pH? Show the reaction of a residue with sodium hydroxide. (Hint: Draw a salt bridge) Complete the following table of the rates of reaction of catalase (the enzyme) with hydrogen neroxide (the substrate). Express in fraction form the ratio of Volume A/Volume B. Express in fraction form the ratio of drops of substrate in A/drops of substrate in B. How do the fractions compare? 3.43.5 Compare the volume of O2 collected at 0C to the standard (35C) Explain the difference