hello i need help to compleate this work, please be natural in your answer without using plagiarism

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BIO220 Exam One: Part Two Short Answer (30 points) Instructions: Answer ALL the following short answer questions IN YOUR OWN WORDS. Your responses must be typed but bulleting, listing, or drawing are acceptable; everything must be legible. Only your class notes/ textbook may be used, if necessary. Answers should not have any citations or sources and will be submitted via Bb to a plagiarism checker before being graded. Submit your ANSWERS only. 1. An otherwise healthy 8-month-old male was brought to a local hospital with a one-week history of dehydration, lethargy, and constipation. Upon admission, he was referred to the pediatric neurology team due to noted \"floppiness\" in his limbs, \"droopiness\" in his eyes, and cyanosis. According to his mother, his diet included breast milk, pureed fresh fruits/vegetables, and raw honey. A full blood count (FBC) revealed neutropenia, indicating an invasive bacterial infection. He was started on penicillin antibiotics and underwent a comprehensive septic workup. However, despite adequate rehydration and antimicrobial therapy, his clinical status deteriorated, exhibiting a decreased respiratory rate requiring intubation and mechanical ventilation. Blood testing for bacterial exotoxin yielded a positive result - Gram-positive Clostridium botulinum, the causative agent of foodborne botulism. This exotoxin is released and spreads when bacterial cells reproduce and are lysed (damaged/ killed). The CDC was notified about the case and was sent to investigate. Define the terms sign and symptom and indicate at least three of each from the case study above. (5 points) 2 Name and describe the etiologic steps the CDC could use to determine which food contained the same causative agent that was found in the patient's system. Why might this bacterium be difficult to culture and identify once ingested? (Hint: What category of microbial disease is botulism). (5 points) The CDC determined that the honey contained the same strain of C. botulinum found in the patient. During testing, they determined that when the honey was heated above 100F, the C. botulinum and its toxins survived. How were the bacteria still able to cause infection after heating? Explain the process/ structures involved. (5 points) Botulism treatment usually includes administering antitoxin, inducing vomiting, and ventilator use, but no antibiotics. Describe the basic steps that Gram-positive C. botulinum would use to build/repair its cell wall and explain why using a cell wall inhibitor like penicillin would not be selectively toxic to the host. (5 points) C. botulinum is an anaerobic peritrichous diplobacillus. o Describe/ draw what its surface morphology would look like under magnification. (2 points) o If its environment suddenly became oxygenated, explain how would it move towards a more suitable environment. (3 points) 2. Portable restrooms (\"Porta-potties\") often contain a blue dye - biocidal disinfectants (didecyldimethylammonium chloride & polyhexamethylene guanidine), fragrance, and surfactants. These chemicals are used to neutralize waste and odor within the restroom but are highly toxic when discharged to landfills and wastewater treatment plants as biocide- contaminated fecal sludge (FS). After analyzing the FS, researchers found that the common opportunistic fecal bacterium, Alcaligenes faecalis, is resistant to the biocidal agents and able to detoxify them in aerobic and anaerobic conditions. They propose using the bacterium to remove to biocides. Based on the information provided, what type of bioremediation is being considered? Provide at least 2 specific pros and 2 specific cons of using this bioremediation method. As the local microbiologist, propose at least one alternative bioremediation method. (5 points)