Question: Henderson Hasselbach question: Consider a simple pH indicator dye that changes color with pH . Let's suppose that our dye absorbs strongly at a given

Henderson Hasselbach question:
Consider a simple pH indicator dye that changes color with pH. Let's suppose that our dye absorbs strongly at a given wavelength at pH 12 while it has essentially no absorbance at pH 2 as shown in the table below
dye concentration pH Abs
10 micromolar 20.00
10 micromolar 120.90
At pH 7 you observe the absorbance of your 10 micromolar solution to be 0.3
Calculate the pKa to 2 significant figures
You can assume that our dye is a simple monoprotic acid that is fully deprotonated at pH 12 and fully protonated at pH 2.

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