Question: Henderson Hasselbach question: Consider a simple pH indicator dye that changes color with pH . Let's suppose that our dye absorbs strongly at a given
Henderson Hasselbach question:
Consider a simple pH indicator dye that changes color with pH Let's suppose that our dye absorbs strongly at a given wavelength at pH while it has essentially no absorbance at pH as shown in the table below
dye concentration pH Abs
micromolar
micromolar
At pH you observe the absorbance of your micromolar solution to be
Calculate the pKa to significant figures
You can assume that our dye is a simple monoprotic acid that is fully deprotonated at pH and fully protonated at pH
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